STRUCTURAL ELUCIDATION OF THE O-ANTIGEN LIPOPOLYSACCHARIDE FROM 2 STRAINS OF PLESIOMONAS-SHIGELLOIDES THAT SHARE A TYPE-SPECIFIC ANTIGEN WITH SHIGELLA-FLEXNERI-6, AND THE COMMON GROUP-1 ANTIGEN WITH SHIGELLA-FLEXNERI SPP AND SHIGELLA-DYSENTERIAE-1

Sugar and methylation analyses of native polysaccharides together with one-dimensional H-1- and C-13- NMR spectroscopy revealed that the two polysaccharides from strains 22074 and 12254 of Plesiomonas shigelloides are identical. The structure of the polysaccharide from strain 22074 was deduced from a uronic acid degradation and by NMR spectroscopy where heteronuclear multiple bond connectivity and tyro-dimensional nuclear Overhauser effect spectroscopy experiments established the pentasaccharide repeating unit: as -->4)-alpha-D-GalpA-(1-->3)-alpha-D-GlcpNAc-(1-->3)-alpha-L-(Rhap-(1-->2)-alpha-L-Rhap-(1-->2)-alpha-L- Rhap-(1-->.