DETECTION OF NEOSPORA-CANINUM IN TISSUE-SECTIONS USING A MURINE MONOCLONAL-ANTIBODY

被引：58
作者：
COLE, RA
LINDSAY, DS
DUBEY, JP
BLAGBURN, BL
机构：
[1] Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn
[2] Zoonotic Diseases Laboratory, Livestock and Poultry Sciences Institute, Beltsville Agricultural Research Center, Agricultural Research Service, Department of Agriculture, Beltsville
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 04期
关键词：
D O I：
10.1177/104063879300500413
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A murine monoclonal antibody (MAb 6G7), isotype IgG2a, produced against tachyzoites of Neospora caninum (isolate NC-1) reacted specifically with tachyzoites of N. caninum in an indirect immunofluorescent antibody test. MAb 6G7 did not react with tachyzoites of Toxoplasma gondii, sporozoites of Isospora suis, Eimeria bovis, or E. tenella, or merozoites of E. bovis in the indirect immunofluorescent antibody test. MAb 6G7 reacted positively with both tachyzoites and bradyzoites of N. caninum in an avidin-biotin peroxidase complex immunohistochemical test on formalin-fixed paraffin-embedded tissues. No reaction was observed with the following: tachyzoites and bradyzoites of T. gondii, T. gondii-like parasites, or Hammondia hammondi; bradyzoites of Frenkelia microti; schizonts and merozoites of Sarcocystis-like organisms; schizonts, sarcocysts, and oocysts/sporocysts of S. cruzi; schizonts and merozoites of S. canis; schizonts of S. hirsuta, S. tenella, and S. capracanis; merozoites of S. neurona and S. neurona-like organisms, E. bovis, or Haemaproteus sp.; bradyzoites and merozoites of S. montanaensis; bradyzoites of S. odocoileocanis, S. cruzi, and S. tenella; meronts, sexual stages, and caryocysts of Caryospora sp. and C. bigenetica; micromerozoites, macromerozoites, and schizonts of Hepatozoon canis; sporozoites, sexual stages, and oocysts of Cryptosporidium parvum and C. baileyi; trophozoites of Monocystis lumbrici, Tritrichomonas foetus, and Balantidium coli; tissue cysts and bradyzoites of Besnoitia sp. and B. jellisoni; amastigotes of Leishmania sp.; and trophic theronts of Ichthyopthirius multifilis. MAb 6G7 reacted with tachyzoites and bradyzoites of N. caninum in natural and experimental infections in dogs, cattle, mice, rats, sheep, and goats, indicating that host origin of the tissues did not affect the performance of the test.
引用
收藏
页码：579 / 584
页数：6
相关论文
共 71 条
  • [1] Anderson M.L., Barr B.C., Blanchard P.C., Neospora-like protozoan infection as a major cause of abortion in California dairy cattle, J Am Vet Med, 198, pp. 241-244, (1991)
  • [2] Anderson M.L., Blanchard P.C., Barr B.C., Hoffman R.L., A survey of causes of bovine abortion occurring in California San Joaouin Valley, J Vet Diagn Invest, 2, pp. 283-287, (1990)
  • [3] Barr B.C., Anderson M.L., Dubey J.P., Conrad P.A., Neospora-like protozoal infections associated with bovine abortions, Vet Pathol, 28, pp. 110-116, (1991)
  • [4] Barr B.C., Anderson M.L., Woods L.W., Neospora-like protozoal infections associated with abortion in goats, J Vet Diagn Invest, 4, pp. 365-367, (1992)
  • [5] Barr B.C., Conrad P.A., Dubey J.P., Anderson M.L., Neospora-like encephalomyelitis in a calf: Pathology, ultrastructure, and immunoreactivity, J Vet Diagn Invest, 3, pp. 39-46, (1991)
  • [6] Bjerkas I., Dubey J.P., Evidence that Neospora caninum is identical to the Toxoplasma-like parasite of Norwegian dogs, Acta Vet Stand, 32, pp. 407-410, (1991)
  • [7] Bjerkas I., Presthus J., Immuno-histochemical and ultrastructural characteristics of a cyst-forming sporozoon associated with encephalomyelitis and myositis in dogs, Acta Pathol Microbiol Immunol Stand, 96, pp. 445-454, (1988)
  • [8] Bontemps M., Yvore P., Technique de purification de suspensions de sporozoites d'Eimeria sur colonee de fibres synthetiques, Ann Rech Vet, 5, pp. 109-113, (1974)
  • [9] Cochrane S., Dubey J.P., Fatal neosporosis in a Golden Retriever dog from Ontario Canada, Can Vet J, 34, pp. 232-233, (1992)
  • [10] Cudden P., Lin D.S., Bowman D.D., Neospora caninum infection in English Springer Spaniel littermates
12345678