THE CHROMOSOME COMPLEMENT OF ERYSIPHE-GRAMINIS F-SP HORDEI ANALYZED BY LIGHT-MICROSCOPY AND FIELD INVERSION GEL-ELECTROPHORESIS

被引：16
作者：
BORBYE, L
LINDELAURSEN, I
CHRISTIANSEN, SK
GIESE, H
机构：
[1] Plant Biology Section, Risø National Laboratory, Roskilde
来源：
MYCOLOGICAL RESEARCH | 1992年 / 96卷
关键词：
D O I：
10.1016/S0953-7562(09)80922-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Light microscopy studies of somatic barley powdery mildew fungal cells at metaphase revealed seven to eight chromosomes of at least three different sizes. Separation of fungal chromosomal DNA by field inversion gel electrophoresis (FIGE) was achieved using a combination of very short and long pulses. FIGE showed distinct bands representing chromosomal DNA, mitochondrial DNA and extrachromosomal plasmid DNA. By combining the results from the two techniques used, the haploid genome of the barley powdery mildew fungus is found to contain five very small chromosomes (0.2-0.8 Mb) and a group of at least two larger chromosomes.
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页码：97 / 102
页数：6
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