DETERMINATION OF CYANIDE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING POSTCOLUMN DERIVATIZATION WITH O-PHTHALALDEHYDE

被引：24

作者：

SUMIYOSHI, K ^{[1
]}

YAGI, T ^{[1
]}

NAKAMURA, H ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,DEPT ANALYT CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 690卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)00976-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, highly sensitive and selective method for the determination of cyanide ion by high-performance liquid chromatography (HPLC) with postcolumn fluorescence derivatization is described. Cyanide ion was separated on an ion-exclusion chromatographic column and derivatized with two reagents, ammonium ion in the mobile phase and o-phthalaldehyde in the postcolumn reagent solution at pH 9.5. Sulfite ion, 2-mercaptoethanol and 3-mercaptopropionic acid did not interfere with the analysis. The average recovery of cyanide from spiked environmental water samples (river water) was 99.0% (n = 6). The relative standard deviation was 2.5% (based on peak area) for cyanide standard solutions (0.1 mu g/ml, n = 10). The linear determination range was from 2.5 ng/ml to 1 mu g/ml. The detection limit was 0.1 ng/ml (signal-to-noise ratio = 3, injection volume 200 mu l).

引用

页码：77 / 82

页数：6

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