共 11 条
[11]
WIELAND DM, 1980, J NUCL MED, V21, P349
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF METAIODOBENZYLGUANIDINE IN WHOLE-BLOOD AND PLASMA OF CANCER-PATIENTS
被引：4
作者：
WAFELMAN, AR
KONINGS, MCP
ROSING, H
HOEFNAGEL, CA
TAAL, BG
MAES, RAA
BEIJNEN, JH
机构：
[1] NETHERLANDS CANC INST,DEPT NUCL MED,1066 CX AMSTERDAM,NETHERLANDS
[2] UNIV UTRECHT,FAC PHARM,DEPT PHARMACEUT ANAL & TOXICOL,3584 CA UTRECHT,NETHERLANDS
[3] NETHERLANDS CANC INST,DEPT MED ONCOL,1066 CX AMSTERDAM,NETHERLANDS
关键词：
CANCER PATIENTS;
HPLC;
MIBG;
PLASMA;
WHOLE BLOOD;
D O I：
10.1016/0731-7085(94)E0034-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ;
081704 ;
摘要：
An accurate and simple high-performance liquid chromatographic (HPLC) assay is presented for the quantitative determination of metaiodobenzylguanidine (MIBG) in human whole blood and plasma. The sample pretreatment involves a solid-phase extraction on Bakerbond SPE cyano columns. The HPLC system comprises a mu Bondapak C-18 column and ammonium phosphate (pH 4.0; 25 mM)-acetonitrile (80:20, v/v) as the mobile phase. Detection is performed by UV absorbance measurements at 254 nm. Linear regression with weighting factor 1/x(2) yielded the smallest sum of per cent relative concentration residuals over the concentration range of the assay (0.1-10 mu g ml(-1)). MIBG levels, at the end of a 3-h infusion, in whole blood and plasma of carcinoid patients were measured and compared with the results obtained with radiodetection after addition of iodine-131-labelled MIBG.
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页码：1173 / 1179
页数：7
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