ENHANCEMENT OF HUMAN NEUTROPHIL FUNCTIONS BY A MONOCLONAL-ANTIBODY DIRECTED AGAINST A 19-KDA ANTIGEN

被引:0
|
作者
MOLLINEDO, F
BURGALETA, C
VELASCO, G
ARROYO, AG
ACEVEDO, A
BARASOAIN, I
机构
[1] HOSP RAMON Y CAJAL,SECC FISIOPATOL LEUCOCITARIA IZQ3,SERV HEMATOL,E-28034 MADRID,SPAIN
[2] HOSP PRINCESA,SERV ANAT PATOL,E-28006 MADRID,SPAIN
来源
JOURNAL OF IMMUNOLOGY | 1992年 / 149卷 / 01期
关键词
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A mouse IgG mAb termed P1C3 was raised against A23187-treated human peripheral blood neutrophils and has been shown to recognize an Ag with an apparent molecular mass of 19 kDa, herein named p19. This p19 Ag was weakly expressed at the cell surface of resting human peripheral blood neutrophils and monocytes, but its cell surface expression was dramatically increased upon activation of these cell types with different secretagogues, including FMLP, PMA, and the calcium ionophores A23187 and ionomycin. A large latent pool of p19 molecules became accessible by immunofluorescence flow cytometry after cell permeabilization of resting neutrophils. A practically total translocation of the intracellular pool of this p19 molecule to the plasma membrane was achieved under appropriate cell stimulation, which induced an almost total degranulation of neutrophil secretory granules. The p19 Ag was absent from platelets, PBL, as well as from the human promyelocytic cell line HL-60, the human promonocytic cell line U937, and the human lymphoid cell lines Daudi and Jurkat. The p19 Ag was also expressed by circulating and/or interstitial neutrophils and monocytes in distinct tissues examined. The mAb P1C3 was found to enhance several neutrophil responses, such as chemotaxis, cell adhesion, phagocytosis, and respiratory burst. These data indicate that the mAb P1C3 recognizes an intracellular Ag in human resting mature neutrophils and monocytes, which upon cell activation is translocated to the cell surface and is able to affect cell functionality.
引用
收藏
页码:323 / 330
页数:8
相关论文
共 50 条
  • [31] IDENTIFICATION AND CHARACTERIZATION OF A TARGET ANTIGEN OF A MONOCLONAL-ANTIBODY DIRECTED AGAINST EIMERIA-TENELLA MEROZOITES
    KO, C
    SMITH, CK
    MCDONELL, M
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 41 (01) : 53 - 63
  • [32] IDENTIFICATION OF HUMAN ERYTHROID PROGENITORS BY A MONOCLONAL-ANTIBODY AGAINST A FETAL ERYTHROCYTE ANTIGEN
    VINCI, G
    EDELMAN, P
    VAINCHENKER, W
    HENRI, A
    MIGLIERINA, R
    ROUGER, P
    REVIRON, J
    BRETONGORIUS, J
    SUREAU, C
    EDELMAN, L
    NOUVELLE REVUE FRANCAISE D HEMATOLOGIE, 1985, 27 (05): : 341 - 341
  • [33] MONOCLONAL-ANTIBODY AGAINST A NUCLEAR MATRIX ANTIGEN IN PROLIFERATING HUMAN-CELLS
    PHILIPOVA, RN
    ZHELEV, NZ
    TODOROV, IT
    HADJIOLOV, AA
    BIOLOGY OF THE CELL, 1987, 60 (01) : 1 - 8
  • [34] A MONOCLONAL-ANTIBODY DIRECTED AGAINST AN ANTIGEN ASSOCIATED WITH CHRONIC LYMPHATIC-LEUKEMIA - STUDIES ON SPECIFICITY
    AGEE, JF
    FAGUET, GB
    JOURNAL OF LEUKOCYTE BIOLOGY, 1985, 38 (01) : 80 - 80
  • [35] MONOCLONAL-ANTIBODY AGAINST HUMAN SQUAMOUS-CELL-CARCINOMA-ASSOCIATED ANTIGEN
    TATAKE, RJ
    AMIN, KM
    MANIAR, HS
    JAMBHEKAR, NA
    SRIKHANDE, SS
    GANGAL, SG
    INTERNATIONAL JOURNAL OF CANCER, 1989, 44 (05) : 840 - 845
  • [36] SYNGENEIC MONOCLONAL ANTIIDIOTYPE ANTIBODIES AGAINST A MONOCLONAL-ANTIBODY TO HUMAN MELANOMA-ASSOCIATED ANTIGEN
    BHATTACHARYACHATTERJEE, M
    MROZEK, E
    CHAKRABORTY, M
    REISFELD, RA
    KOHLER, H
    FOON, KA
    JOURNAL OF IMMUNOLOGY, 1993, 150 (08): : A142 - A142
  • [37] HUMAN MONOCLONAL-ANTIBODY AGAINST A TUMOR-ASSOCIATED URINARY SARCOMA ANTIGEN
    HUTH, JF
    SAXTON, RE
    MORTON, DL
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1986, 27 : 339 - 339
  • [38] A MONOCLONAL-ANTIBODY DIRECTED AGAINST A HUMAN CELL-MEMBRANE ANTIGEN PREVENTS CELL SUBSTRATE ADHESION AND TUMOR INVASION
    DEPOTTER, CR
    SCHELFHOUT, AM
    DESMET, FH
    VANDAMME, S
    DERIDDER, L
    DHONT, E
    VANEMMELO, J
    AMERICAN JOURNAL OF PATHOLOGY, 1994, 144 (01): : 95 - 103
  • [39] A MURINE MONOCLONAL-ANTIBODY DIRECTED AGAINST THE GERBICH-3 BLOOD-GROUP ANTIGEN
    LOIRAT, MJ
    GOURBIL, A
    FRIOUX, Y
    MULLER, JY
    BLANCHARD, D
    VOX SANGUINIS, 1992, 62 (01) : 45 - 48
  • [40] USE OF MONOCLONAL-ANTIBODY AGAINST HUMAN NEUTROPHIL ELASTASE IN NORMAL AND LEUKEMIC MYELOID CELLS
    PULFORD, KAF
    ERBER, WN
    CRICK, JA
    OLSSON, I
    MICKLEM, KJ
    GATTER, KC
    MASON, DY
    JOURNAL OF CLINICAL PATHOLOGY, 1988, 41 (08) : 853 - 860