PHASE ACCURACY IN HIGH-RESOLUTION ELECTRON-MICROSCOPY OF TRIGONAL AND ORTHORHOMBIC PURPLE MEMBRANE

被引:14
作者
BULLOUGH, PA [1 ]
HENDERSON, R [1 ]
机构
[1] MRC, MOLEC BIOL LAB, CAMBRIDGE CB2 2QH, ENGLAND
关键词
D O I
10.1016/S0006-3495(90)82413-9
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
High-resolution images of orthorhombic purple membrane have been obtained by electron cryomicroscopy with spot-scan illumination, and the projection structure at 3.9 Å resolution calculated after image processing and averaging of the data. Since the phases of the structure factors in the projection down the orthorhombic twofold axis should be either 0 or 180°, this offers the first opportunity to make an independent test of the estimated accuracy of high-resolution phases obtained by electron microscopy. The results show the final phases are less accurate than previously estimated by a small factor (1.3). Careful comparison of the new orthorhombic structure to the known trigonal structure shows only small differences after account is taken of a slight difference in the tilt angle of the molecules in the two crystals. This is consistent with the available kinetic and spectroscopic data which show very small differences in behavior. © 1990, The Biophysical Society. All rights reserved.
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收藏
页码:705 / 711
页数:7
相关论文
共 25 条
[1]  
BALDWIN J, 1984, ULTRAMICROSCOPY, V14, P319, DOI 10.1016/0304-3991(84)90217-1
[2]   IMAGES OF PURPLE MEMBRANE AT 2.8 A RESOLUTION OBTAINED BY CRYO-ELECTRON MICROSCOPY [J].
BALDWIN, JM ;
HENDERSON, R ;
BECKMAN, E ;
ZEMLIN, F .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 202 (03) :585-591
[3]  
BALDWIN JM, 1987, CRYSTALLOGRAPHY MOL, P101
[4]   THE TREATMENT OF ERRORS IN THE ISOMORPHOUS REPLACEMENT METHOD [J].
BLOW, DM ;
CRICK, FHC .
ACTA CRYSTALLOGRAPHICA, 1959, 12 (10) :794-802
[5]   GEOMETRIC SOURCES OF REDUNDANCY IN INTENSITY DATA AND THEIR USE FOR PHASE DETERMINATION [J].
BRICOGNE, G .
ACTA CRYSTALLOGRAPHICA SECTION A, 1974, A 30 (MAY1) :395-405
[6]   METHODS AND PROGRAMS FOR DIRECT-SPACE EXPLOITATION OF GEOMETRIC REDUNDANCIES [J].
BRICOGNE, G .
ACTA CRYSTALLOGRAPHICA SECTION A, 1976, 32 (SEP1) :832-847
[7]   USE OF SPOT-SCAN PROCEDURE FOR RECORDING LOW-DOSE MICROGRAPHS OF BEAM-SENSITIVE SPECIMENS [J].
BULLOUGH, P ;
HENDERSON, R .
ULTRAMICROSCOPY, 1987, 21 (03) :223-229
[8]  
BULLOUGH PA, 1990, IN PRESS ELECTRON MI
[9]  
BULLOUGH PA, 1990, IN PRESS J MOL BIOL
[10]   CRYSTAL STRUCTURE OF MYOGLOBIN - PHASE DETERMINATION TO A RESOLUTION OF 2A BY METHOD OF ISOMORPHOUS REPLACEMENT [J].
DICKERSON, R ;
KENDREW, JC ;
STRANDBERG, BE .
ACTA CRYSTALLOGRAPHICA, 1961, 14 (11) :1188-&