CRESYL VIOLET - A RED FLUORESCENT NISSL STAIN

被引：44

作者：

ALVAREZBUYLLA, A

LING, CY

KIRN, JR

机构：

[1] The Rockefeller University, New York, NY 10021

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 33卷 / 2-3期

关键词：

Counterstain; Cytoarchitecture; Fluorescence; Fluorescent tracer; Fluoro-Gold; Histology; Neurons;

D O I：

10.1016/0165-0270(90)90016-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy. © 1990.

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页码：129 / 133

页数：5

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