CRESYL VIOLET - A RED FLUORESCENT NISSL STAIN

被引：44
|
作者：
ALVAREZBUYLLA, A
LING, CY
KIRN, JR
机构：
[1] The Rockefeller University, New York, NY 10021
来源：
JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 33卷 / 2-3期
关键词：
Counterstain; Cytoarchitecture; Fluorescence; Fluorescent tracer; Fluoro-Gold; Histology; Neurons;
D O I：
10.1016/0165-0270(90)90016-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy. © 1990.
引用
收藏
页码：129 / 133
页数：5
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