AN IN-VIVO STUDY OF THE EFFECTS OF ISCHEMIA ON UTERINE CONTRACTION, INTRACELLULAR PH AND METABOLITES IN THE RAT

1. There are no data concerning the functional or metabolic effects of hypoxia in, vivo in smooth muscle. We have therefore used P-31-NMR spectroscopy and intra-uterine pressure measurements to examine simultaneously, in vivo, the effect of ischaemia on uterine metabolites, intracellular pH (pH(i)) and force. 2. A 1-2 cm portion of uterus from day 1 postpartum anaesthetized rats was exteriorized and an NMR surface coil placed on it. A balloon catheter in the uterine lumen recorded intra-uterine pressure changes from the same area. Reversible occluders were placed around the uterine artery. 3. Occlusion produced a decrease and then abolition of contractions, within 10 min. In four of five animals contraction was abolished within 2 min. Upon reperfusion force was rapidly restored (1 min), in all preparations. The mean level of force was significantly above control (pre-occlusion) 20-30 min after reperfusion. 4. The NMR data showed a significant fall in [ATP] (28%) and [phosphocreatine] (34%) during occlusion. Inorganic phosphate doubled in concentration during this period. Metabolites recovered slowly upon reperfusion, taking 20-30 min to return to preocclusion levels. 5. The mean pH(i) fell from 7.32 to 7.00 upon occlusion and Was rapidly reversed upon reperfusion. The changes in pH(i) closely correlated With the changes in uterine force. Decreases of pH(i) of a similar magnitude in, vitro have previously been shown to abolish contractions; thus it is suggested that during ischaemia in vivo the depression of contraction is caused by the large fall in pH(i).