TRANSCRIPTION INITIATION-COMPLEXES AND UPSTREAM ACTIVATION WITH RNA POLYMERASE-II LACKING THE C-TERMINAL DOMAIN OF THE LARGEST SUBUNIT

被引：49
作者：
BURATOWSKI, S
SHARP, PA
机构：
[1] MIT,CTR CANC RES,CAMBRIDGE,MA 02139
[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
来源：
MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 10期
关键词：
D O I：
10.1128/MCB.10.10.5562
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
RNA polymerase II assembles with other factors on the adenovirus type 2 major late promoter to generate pairs of transcription initiation complexes resolvable by nondenaturing gel electrophoresis. The pairing of the complexes is caused by the presence or absence of the C-terminal domain of the largest subunit. This domain is not required for transcription stimulation by the major late transcription factor in vitro.
引用
收藏
页码：5562 / 5564
页数：3
相关论文
共 18 条
[1]   THE C-TERMINAL DOMAIN OF THE LARGEST SUBUNIT OF RNA POLYMERASE-II OF SACCHAROMYCES-CEREVISIAE, DROSOPHILA-MELANOGASTER, AND MAMMALS - A CONSERVED STRUCTURE WITH AN ESSENTIAL FUNCTION [J].
ALLISON, LA ;
WONG, JKC ;
FITZPATRICK, VD ;
MOYLE, M ;
INGLES, CJ .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (01) :321-329
[2]   MUTATIONS IN RNA POLYMERASE-II ENHANCE OR SUPPRESS MUTATIONS IN GAL4 [J].
ALLISON, LA ;
INGLES, CJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2794-2798
[3]   EXTENSIVE HOMOLOGY AMONG THE LARGEST SUBUNITS OF EUKARYOTIC AND PROKARYOTIC RNA-POLYMERASES [J].
ALLISON, LA ;
MOYLE, M ;
SHALES, M ;
INGLES, CJ .
CELL, 1985, 42 (02) :599-610
[4]   GENETIC-ANALYSIS OF THE REPETITIVE CARBOXYL-TERMINAL DOMAIN OF THE LARGEST SUBUNIT OF MOUSE RNA POLYMERASE-II [J].
BARTOLOMEI, MS ;
HALDEN, NF ;
CULLEN, CR ;
CORDEN, JL .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (01) :330-339
[5]   IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS [J].
BLUM, H ;
BEIER, H ;
GROSS, HJ .
ELECTROPHORESIS, 1987, 8 (02) :93-99
[6]   5 INTERMEDIATE COMPLEXES IN TRANSCRIPTION INITIATION BY RNA POLYMERASE-II [J].
BURATOWSKI, S ;
HAHN, S ;
GUARENTE, L ;
SHARP, PA .
CELL, 1989, 56 (04) :549-561
[7]  
CADENA DL, 1987, J BIOL CHEM, V262, P12468
[8]   AN RNA POLYMERASE-II TRANSCRIPTION FACTOR BINDS TO AN UPSTREAM ELEMENT IN THE ADENOVIRUS MAJOR LATE PROMOTER [J].
CARTHEW, RW ;
CHODOSH, LA ;
SHARP, PA .
CELL, 1985, 43 (02) :439-448
[9]   A UNIQUE STRUCTURE AT THE CARBOXYL TERMINUS OF THE LARGEST SUBUNIT OF EUKARYOTIC RNA POLYMERASE-II [J].
CORDEN, JL ;
CADENA, DL ;
AHEARN, JM ;
DAHMUS, ME .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (23) :7934-7938
[10]   PURIFICATION USING POLYETHYLENIMINE PRECIPITATION AND LOW-MOLECULAR WEIGHT SUBUNIT ANALYSES OF CALF THYMUS AND WHEAT-GERM DNA-DEPENDENT RNA POLYMERASE-II [J].
HODO, HG ;
BLATTI, SP .
BIOCHEMISTRY, 1977, 16 (11) :2334-2343
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