PREVALENCE OF TRIMETHOPRIM-RESISTANT DIHYDROFOLATE-REDUCTASE GENES IDENTIFIED WITH OLIGONUCLEOTIDE PROBES IN PLASMIDS FROM ISOLATES OF COMMENSAL FECAL FLORA

被引:16
作者
ADRIAN, PV
KLUGMAN, KP
AMYES, SGB
机构
[1] UNIV EDINBURGH, SCH MED, DEPT MED MICROBIOL, EDINBURGH EH8 9AG, MIDLOTHIAN, SCOTLAND
[2] UNIV WITWATERSRAND, DEPT MED MICROBIOL, JOHANNESBURG, SOUTH AFRICA
[3] S AFRICAN INST MED RES, JOHANNESBURG 2000, SOUTH AFRICA
基金
英国惠康基金;
关键词
D O I
10.1093/jac/35.4.497
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In a recent survey of trimethoprim resistance, 357 Gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in South Africa. Trimethoprim resistance was transferable in 184 (52%) of the isolates. A further 12 (3%) transferred in the presence of an X(+) factor. The transconjugants were probed with intragenic oligonucleotide probes for the type Ia, Ib, IIIa, VIII, V, VI, VII, IX, X and XII dihydrofolate reductase genes. Contrary to all previous data, the most prevalent dihydrofolate reductase gene was the type Ib (30%) followed by the type VIII (23%), V (13%), Ia (6%), VII (3%) and XII (0.5%). None of the strains hybridised to the type IIIa, XI, X and the VI dihydrofolate reductase probes. Plasmid restriction profiles revealed that the high prevalence of the type Ib and VIII dihydrofolate reductase genes resulted from the presence of ubiquitous plasmids. These results highlight the previous problems associated with the distinction of closely related dihydrofolate reductase genes.
引用
收藏
页码:497 / 508
页数:12
相关论文
共 44 条
[1]   TRIMETHOPRIM RESISTANCE IN SOUTH-AFRICAN ISOLATES OF AEROBIC GRAM-NEGATIVE FECAL FLORA [J].
ADRIAN, PV ;
KOORNHOF, HJ ;
WYLIE, BA .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1993, 12 (12) :916-921
[2]   TRIMETHOPRIM RESISTANCE PLASMIDS IN FECAL BACTERIA [J].
AMYES, SGB ;
GOULD, IM .
ANNALES DE MICROBIOLOGIE, 1984, B135 (02) :177-186
[3]   THE TYPE-VII DIHYDROFOLATE-REDUCTASE - A NOVEL PLASMID-ENCODED TRIMETHOPRIM-RESISTANT ENZYME FROM GRAM-NEGATIVE BACTERIA ISOLATED IN BRITAIN [J].
AMYES, SGB ;
TOWNER, KJ ;
CARTER, GI ;
THOMSON, CJ ;
YOUNG, HK .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1989, 24 (02) :111-119
[4]   R-FACTOR TRIMETHOPRIM RESISTANCE MECHANISM - INSUSCEPTIBLE TARGET SITE [J].
AMYES, SGB ;
SMITH, JT .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1974, 58 (02) :412-418
[5]   NOVEL DIHYDROFOLATE REDUCTASES ISOLATED FROM EPIDEMIC STRAINS OF TRIMETHOPRIM SULFAMETHOXAZOLE-RESISTANT SHIGELLA-SONNEI [J].
BARG, NL ;
HUTSON, FS ;
WHEELER, LA ;
THOMSON, CJ ;
AMYES, SGB ;
WHARTON, M ;
SCHAFFNER, W .
JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (02) :466-473
[6]   SEQUENCE IDENTITY WITH TYPE-VIII AND ASSOCIATION WITH IS176 OF TYPE-IIIC DIHYDROFOLATE-REDUCTASE FROM SHIGELLA-SONNEI [J].
BARG, NL ;
REGISTER, S ;
THOMSON, C ;
AMYES, S .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (01) :112-116
[7]  
BRUMFITT W, 1971, LANCET, V1, P315
[8]  
BURCHALL JJ, 1965, MOL PHARMACOL, V1, P126
[9]   THE DISTRIBUTION OF THE DHFR GENES IN TRIMETHOPRIM-RESISTANT URINARY-TRACT ISOLATES FROM TAIWAN [J].
CHANG, LL ;
CHANG, SF ;
CHOW, TY ;
WU, WJ ;
CHANG, JC .
EPIDEMIOLOGY AND INFECTION, 1992, 109 (03) :453-462
[10]   MUTANTS OF ESCHERICHIA-COLI REQUIRING METHIONINE OR VITAMIN-B12 [J].
DAVIS, BD ;
MINGIOLI, ES .
JOURNAL OF BACTERIOLOGY, 1950, 60 (01) :17-28