DETECTION OF OLIGONUCLEOTIDE DUPLEX FORMS BY ION-SPRAY MASS-SPECTROMETRY

被引：126

作者：

GANEM, B ^{[1
]}

LI, YT ^{[1
]}

HENION, JD ^{[1
]}

机构：

[1] CORNELL UNIV,NEW YORK STATE COLL VET MED,ITHACA,NY 14850

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 09期

关键词：

D O I：

10.1016/S0040-4039(00)60314-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Three representative examples of duplex DNA, each eight base pairs in length, have been examined by ion-spray mass spectrometry. The ability to detect base-paired duplex ions in each case further indicates the use of this technique for monitoring noncovalent interactions in biological systems.

引用

页码：1445 / 1448

页数：4

共 11 条

[1] DIRECT OBSERVATION OF A TERNARY COMPLEX BETWEEN THE DIMERIC ENZYME HIV-1 PROTEASE AND A SUBSTRATE-BASED INHIBITOR [J].

BACA, M ;

KENT, SBH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) :3992-3993

[2] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY [J].

BRUINS, AP ;

COVEY, TR ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1987, 59 (22) :2642-2646

[3]

Covey T R, 1988, Rapid Commun Mass Spectrom, V2, P249, DOI 10.1002/rcm.1290021111

[4] ELECTROSPRAY IONIZATION-PRINCIPLES AND PRACTICE [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

MASS SPECTROMETRY REVIEWS, 1990, 9 (01) :37-70

[5] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[6] OBSERVATION OF NONCOVALENT ENZYME SUBSTRATE AND ENZYME PRODUCT COMPLEXES BY ION-SPRAY MASS-SPECTROMETRY [J].

GANEM, B ;

LI, YT ;

HENION, JD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (20) :7818-7819

[7] DETECTION OF NONCOVALENT RECEPTOR LIGAND COMPLEXES BY MASS-SPECTROMETRY [J].

GANEM, B ;

LI, YT ;

HENION, JD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (16) :6294-6296

[8] MASS-SPECTROMETRIC DETECTION OF THE NONCOVALENT GDP-BOUND CONFORMATIONAL STATE OF THE HUMAN H-RAS PROTEIN [J].

GANGULY, AK ;

PRAMANIK, BN ;

TSARBOPOULOS, A ;

COVEY, TR ;

HUANG, E ;

FUHRMAN, SA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (16) :6559-6560

[9] THE CRYSTAL-STRUCTURE OF D(CCCCGGGG) - A NEW A-FORM VARIANT WITH AN EXTENDED BACKBONE CONFORMATION [J].

HARAN, TE ;

SHAKKED, Z ;

WANG, AHJ ;

RICH, A .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1987, 5 (02) :199-217

[10] OBSERVATION OF THE HEME GLOBIN COMPLEX IN NATIVE MYOGLOBIN BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY [J].

KATTA, V ;

CHAIT, BT .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (22) :8534-8535

← 1 2 →