Clinico-pathology, hematology, and biochemistry responses toward Pasteurella multocida Type B:2 via oral and subcutaneous route of infections

被引:10
作者
Chung, Eric Lim Teik [1 ]
Abdullah, Faez Firdaus Jesse [1 ,2 ]
Adamu, Lawan [1 ,3 ]
Marza, Ali Dhiaa [1 ,4 ]
Ibrahim, Hayder Hamzah [1 ,5 ]
Zamri-Saad, Mohd [6 ]
Haron, Abdul Wahid [1 ,2 ]
Saharee, Abdul Aziz [1 ]
Lila, Mohd Azmi Mohd [6 ]
Omar, Abdul Rahman [6 ]
Abu Bakar, Md Zuki [7 ]
Norsidin, Mohd Jefri [1 ]
机构
[1] Univ Putra Malaysia, Fac Vet Med, Dept Vet Clin Studies, Serdang 43400, Malaysia
[2] Univ Putra Malaysia, Res Ctr Ruminant Dis, Dept Ruminant Dis, Serdang 43400, Malaysia
[3] Univ Maiduguri, Fac Vet Med, Dept Vet Med, Serdang 43400, Selangor, Malaysia
[4] Al Qasim Green Univ, Fac Vet Med, Dept Vet Internal Med, Al Qasim, Iraq
[5] Al Furat Alawast Tech Univ, Tech Inst Babil, Dept Vet Med, Kufa, Iraq
[6] Univ Putra Malaysia, Fac Vet Med, Dept Vet Pathol & Microbiol, Serdang 43400, Selangor, Malaysia
[7] Univ Putra Malaysia, Fac Vet Med, Dept Preclin, Serdang 43400, Selangor, Malaysia
关键词
buffalo heifers; clinico-pathology; hematology and biochemistry responses; oral route; Pasteurella multocida Type B:2; subcutaneous route;
D O I
10.14202/vetworld.2015.783-792
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Background: Pasteurella multocida a Gram-negative bacterium has been identified as the causative agent of many economically important diseases in a wide range of hosts. Hemorrhagic septicemia is a disease caused by P. multocida serotype B:2 and E:2. The organism causes acute, a highly fatal septicemic disease with high morbidity and mortality in cattle and more susceptible in buffaloes. Therefore, the aim of this study was to investigate the clinical signs, blood parameters, post mortem and histopathology changes caused by P. multocida Type B:2 infections initiated through the oral and subcutaneous routes. Methods: Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 ml of phosphate buffer saline; Groups 2 and 3 were inoculated with 10 ml of 1012 colony forming unit of P. multocida Type B:2 subcutaneously and orally respectively. Results: There was a significant difference (p<0.05) in temperature between the subcutaneous and the control group. The results revealed significant differences (p< 0.05) in erythrocytes, hemoglobin, packed cell volume, leukocytes, monocytes, and A: G ratio between the subcutaneous and the control group. Furthermore, there were significant differences (p<0.05) in leukocytes, band neutrophils, segmented neutrophils, lymphocytes, eosinophils, basophils, thrombocytes, plasma protein, icterus index, gamma glutamyl tranferase and A: G ratio between the oral and the control group. The post mortem lesions of the subcutaneous group buffaloes showed generalized hyperemia, congestion and hemorrhage of the immune organs, gastrointestinal tract organs and vital organs. The oral group buffaloes showed mild lesions in the lung and liver. Histologically, there were significant differences (p<0.05) in hemorrhage and congestion; necrosis and degeneration; inflammatory cells infiltration; and edema in between the groups. Conclusion: This study was a proof that oral route infection of P. multocida Type B:2 can be used to stimulate host cell responses where oral vaccine through feed can be developed in the near future.
引用
收藏
页码:783 / 792
页数:10
相关论文
共 30 条
[1]  
Abubakar M.S., 2011, BASIC APPL PATHOL, V4, P130, DOI DOI 10.1111/j.1755-9294.2011.01113.x
[2]  
Abubakar M, 2012, PAK VET J, V32, P147
[3]  
Ali O.S., 2014, AM J ANIM VET SCI, V9, P177
[4]  
Annas S., 2014, J VET SCI TECHNOL, V5, P2
[5]   New sites of localisation of Pasteurella multocida B:2 in buffalo surviving experimental haemorrhagic septicaemia [J].
Annas, Salleh ;
Zamri-Saad, Mohammad ;
Jesse, Faez Firdaus Abdullah ;
Zunita, Zakaria .
BMC VETERINARY RESEARCH, 2014, 10
[6]  
[Anonymous], 2010, SCHALMS VET HEMATOLO
[7]  
CARTER GR, 1962, CAN J COMP MED VET S, V26, P238
[8]  
De Alwis M. C. L., 1999, 22310916 EISSN ACIAR, P57
[10]  
DEALWIS MCL, 1992, BRIT VET J, V148, P99, DOI 10.1016/0007-1935(92)90101-6