STRUCTURE DETERMINATION OF CUCUMBER GREEN MOTTLE MOSAIC-VIRUS BY X-RAY FIBER DIFFRACTION - SIGNIFICANCE FOR THE EVOLUTION OF TOBAMOVIRUSES

被引：43
作者：
WANG, H
STUBBS, G
机构：
[1] Department of Molecular Biology, Vanderbilt University, Nashville
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 239卷 / 03期
关键词：
TOBAMOVIRUSES; SUBGROUP; 2; CUCUMBER GREEN MOTTLE MOSAIC VIRUS; FIBER DIFFRACTION; CARBOXYL-CARBOXYLATE PAIRS;
D O I：
10.1006/jmbi.1994.1379
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cucumber green mottle mosaic virus (CGMMV) is a rod-shaped virus of the tobacco mosaic virus (TMV) group. The structure of cucumber green mottle mosaic virus has been determined by fiber diffraction methods at 3:4 Å resolution, and refined by molecular dynamics methods to an R factor of 0.093. Disassembly of TMV is driven by the mutual repulsion of intersubunit carboxyl-carboxylate pairs, but one of these pairs is not Gonserved in CGMMV. An alternative pair, located about 5 Å from the site of the TMV pair, has been found in CGMMV. Comparison of the two structures suggests that the carboxylate groups are free to migrate in the subunit interfaces during evolution. © 1994 Academic Press Limited.
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页码：371 / 384
页数：14
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