METABOLISM OF GLUCOSE 1,6-P2 .2. GLUCOSE 1,6-P2 PHOSPHATASE IN PIG MUSCLE

被引：7
作者：
BASSOLS, A
CUSSO, R
CARRERAS, J
机构：
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1985年 / 81卷 / 04期
关键词：
D O I：
10.1016/0305-0491(85)90101-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Most of the glucose, 1,6-P2 [diphosphate] phosphatase activity of pig skeletal muscle is present in the cytosolic fraction. Four peaks of glucose 1,6-P2 phosphatase activity are obtained when the cytosolic fraction from pig muscle is subjected to DE-cellulose chromatography. All the peaks hydrolyze other phosphocompounds in addition to glucose 1,6-P2. The glucose 1,6-P2 phosphatase activity of the main peak shows an optimal neutral pH. It is activated by divalent cations, Mg2+ being more effective than Mn2+. The addition of Ca2+ or EGTA does not affect the enzymatic activity. IMP does not possess any effect. This enzyme is different from the glucose 1,6-P2 phosphatases found in mouse brain cytosol and rat skeletal muscle.
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页码：981 / 987
页数：7
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