MEASUREMENT OF VERY SHORT FLUORESCENCE LIFETIMES BY SINGLE-PHOTON COUNTING

被引:45
作者
HARTIG, PR
SAUER, K
LO, CC
LESKOVAR, B
机构
[1] UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
[2] UNIV CALIF,LAWRENCE LIVERMORE LAB,LIVERMORE,CA 94550
关键词
PHOTODETECTORS;
D O I
10.1063/1.1134834
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Measurement of very shoft fluorescence lifetimes by the single-photon technique is made possible by an improved fluorescence lifetime system. Fluorescence lifetimes of 4. 94 plus or minus 0. 07 nsec for anthracene in cyclohexane, 640 plus or minus 30 psec for diphenyl butadiene in cyclohexane, and 90 plus or minus 30 psec for erythrosin in water were determined. Complications in the analysis of lifetime data introduced by intrinsic fluorescence and phosphorescence processes in commonly used absorption filters are discussed. Certain ″blind spots″ are found in the electronic pulse pileup rejection schemes most commonly used in photon counting.
引用
收藏
页码:1122 / 1129
页数:8
相关论文
共 26 条
[1]  
Alfano R. R., 1972, Optics Communications, V6, P98, DOI 10.1016/0030-4018(72)90203-9
[2]  
BERLMAN IB, 1971, HDB FLUORESCENCE SPE, P356
[4]   CORRECTION FOR PHOTON PILE-UP IN MEASUREMENT OF RADIATIVE LIFETIMES [J].
COATES, PB .
JOURNAL OF PHYSICS E-SCIENTIFIC INSTRUMENTS, 1968, 1 (08) :878-&
[5]   CORRECTION METHODS FOR PHOTON PILE-UP IN LIFETIME DETERMINATION BY SINGLE-PHOTON COUNTING [J].
DAVIS, CC ;
KING, TA .
JOURNAL OF PHYSICS PART A GENERAL, 1970, 3 (01) :101-&
[6]   CORRECTION OF SINGLE PHOTON OR PARTICLE TIMING MEASUREMENTS FOR MULTIPARTICLE EVENTS [J].
DONOHUE, DE ;
STERN, RC .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1972, 43 (05) :791-&
[7]   ANALYSIS OF FLUORESCENCE DECAY CURVES BY MEANS OF LAPLACE TRANSFORMATION [J].
GAFNI, A ;
MODLIN, RL ;
BRAND, L .
BIOPHYSICAL JOURNAL, 1975, 15 (03) :263-280
[8]  
GREENBERG A, 1966, INT S LUMINESCENCE, P71
[9]   IMPROVEMENT OF NANOSECOND FLUORIMETERS TO OVERCOME DRIFT PROBLEMS [J].
HAZAN, G ;
GRINVALD, A ;
MAYTAL, M ;
STEINBERG, IZ .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1974, 45 (12) :1602-1604
[10]   STUDIES ON ANALYSIS OF FLUORESCENCE DECAY DATA BY METHOD OF MOMENTS [J].
ISENBERG, I ;
MULLOOLY, JP ;
DYSON, RD ;
HANSON, R .
BIOPHYSICAL JOURNAL, 1973, 13 (10) :1090-1115