DETERMINATION OF CATECHOLAMINES IN SINGLE ADRENAL-MEDULLARY CELLS BY CAPILLARY ELECTROPHORESIS AND LASER-INDUCED NATIVE FLUORESCENCE

被引：134
作者：
CHANG, HT
YEUNG, ES
机构：
[1] IOWA STATE UNIV SCI & TECHNOL,US DOE,AMES LAB,AMES,IA 50011
[2] IOWA STATE UNIV SCI & TECHNOL,DEPT CHEM,AMES,IA 50011
来源：
ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 06期
关键词：
D O I：
10.1021/ac00102a010
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.
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页码：1079 / 1083
页数：5
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