Human immunodeficiency virus (HIV) reverse transcriptase was used as a sample protein to determine whether the optimum conditions for crystallisation in microgravity were identical to those on Earth. The degree of reproducibility of the results in microgravity was also tested. The results showed that; comparing experiments performed in identical apparatus, the conditions for obtaining the largest crystals in microgravity were different from the optimal conditions on Earth. Crystal form, size, visual quality and the reproducibility of the results were no different from those on Earth. Crystallisation took place in the vapour diffusion set-up of the Russian Kashtan apparatus which flew on a Photon satellite (4-20 October 1991).