POLYMORPHISM IN DNA RESTRICTION PATTERNS OF COXIELLA-BURNETII ISOLATES INVESTIGATED BY PULSED-FIELD GEL-ELECTROPHORESIS AND IMAGE-ANALYSIS

被引：36

作者：

THIELE, D

WILLEMS, H

KOPF, G

KRAUSS, H

机构：

[1] Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen, 6300

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1993年 / 9卷 / 04期

关键词：

COXIELLA-BURNETII; Q-FEVER; PULSED FIELD GEL ELECTROPHORESIS; IMAGE ANALYSIS;

D O I：

10.1007/BF00157400

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Pulsed field gel electrophoresis was used for differentiation of Coxiella burnetii isolates derived from animals in Europe and one from Namibia. Previously published data on isolates from North America demonstrated four groups with distinct banding patterns. Fingerprints of prototype isolates as references confirmed four different cleavage patterns upon NotI restriction of total DNA as described. With isolates available at our institute five additional DNA banding patterns were obtained from European and one Namibian isolate after endonuclease restriction with NotI. To compare patterns from different electrophoretic runs more precisely an image analysis system was used.

引用

页码：419 / 425

页数：7

共 5 条

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