UROKINASE-TYPE PLASMINOGEN-ACTIVATOR IN RABBIT TEARS - COMPARISON WITH HUMAN TEARS

被引：11

作者：

TOZSER, J

BERTA, A

机构：

[1] DEBRECEN UNIV MED,DEPT OPHTHALMOL,POB 29,H-4012 DEBRECEN,HUNGARY

[2] DEBRECEN UNIV MED,DEPT BIOCHEM,H-4012 DEBRECEN,HUNGARY

来源：

EXPERIMENTAL EYE RESEARCH | 1990年 / 51卷 / 01期

关键词：

chromogenic method; fibrin zymography; immunoblotting; plasminogen activator; rabbit tears;

D O I：

10.1016/0014-4835(90)90167-S

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Plasminogen activator activity was determined in stimulated normal rabbit tears, and was identified as being due to urokinase using specific antibodies. The activator activity is about 100-fold higher in rabbit tears than in human tears. The molecular weight of the activator was determined by fibrin zymography to be 40-50 kDa. However, a multiband pattern of urokinase-type antigen ranging in molecular weight from 60-70 kDa to 200 kDa was detected by immunoblotting. The 60-70 kDa protein was resistant to reduction. The antigen concentration is more than 100-fold higher than the concentration expected from the activity measurement, indicating that most of the urokinase in rabbit tears is in inactive form, such as in inhibitor or receptor complexes. © 1990.

引用

页码：33 / 37

页数：5

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