SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI OF A MYCOPLASMA-HOMINIS GENE ENCODING ELONGATION FACTOR-TU

被引：11
|
作者：
LUNEBERG, E
KAMLA, V
HADDING, U
FROSCH, M
机构：
[1] MED HSCH HANNOVER,INST MED MIKROBIOL,KONSTANTY GUTSCHOW STR,W-3000 HANNOVER 61,GERMANY
[2] UNIV DUSSELDORF,INST MED MIKROBIOL & VIROL,W-4000 DUSSELDORF 1,GERMANY
来源：
GENE | 1991年 / 102卷 / 01期
关键词：
RECOMBINANT DNA; GENOMIC LIBRARY; MONOCLONAL ANTIBODY;
D O I：
10.1016/0378-1119(91)90550-U
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclonal antibody. The nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-Da protein. Computer-aided sequence comparison indicated that the gene codes for elongation factor Tu.
引用
收藏
页码：123 / 127
页数：5
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