REDUCTION OF BIOLOGICAL-ACTIVITY OF MURINE RECOMBINANT INTERLEUKIN-1-BETA BY SELECTIVE DEAMIDATION AT ASPARAGINE-149

被引：23
作者：
DAUMY, GO [1 ]
WILDER, CL [1 ]
MERENDA, JM [1 ]
MCCOLL, AS [1 ]
GEOGHEGAN, KF [1 ]
OTTERNESS, IG [1 ]
机构：
[1] PFIZER INC,DIV CENT RES,GROTON,CT 06340
来源：
FEBS LETTERS | 1991年 / 278卷 / 01期
关键词：
INTERLEUKIN-1; CYTOKINE; DEAMIDATION;
D O I：
10.1016/0014-5793(91)80093-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A biologically active preparation of murine recombinant interleukin-1-beta (mIL-1-beta) from Escherichia coli cell lysates contained two forms of mIL-1-beta with pI 8.7 and pI 8.1, respectively. Treatment with 0.1 M Tris, pH 8.5, at 37-degrees-C for 35 h converted the pI 8.7 form to the pI 8.1 form by the selective deamidation of an asparagine residue (Asn149) in the mIL-1-beta molecule. Deamidated mIL-1-beta had 3- to 5-fold lower co-mitogenic activity and receptor affinity than the unmodified form.
引用
收藏
页码：98 / 102
页数：5
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