AN ENZYME-IMMUNOASSAY FOR ATRACTYLOSIDE, THE NEPHROTOXIN OF CALLILEPIS-LAUREOLA (IMPILA)

被引：17
作者：
BYE, SN
COETZER, THT
DUTTON, MF
机构：
[1] Department of Biochemistry, University of Natal, Pietermartizburg
来源：
TOXICON | 1990年 / 28卷 / 08期
关键词：
D O I：
10.1016/0041-0101(90)90030-B
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Tubers of Callilepis laureola, a traditional remedy, contain an inhibitor of oxidative phosphorylation; atractyloside. A "competitive" ELISA was developed, using the antiserum produced to an atractyloside-protein conjugate. An ovalbumin-atractyloside conjugate was adsorbed to microtitre wells and plates incubated with sample (atractyloside or tuber extract) and antiserum. After successive incubation with secondary antibody-enzyme conjugate and substrate, the absorbance was read at 405nm. Antibody working dilution was low, but results, confirmed by thin layer chromatography, indicate the immunoassay has diagnostic potential. © 1990.
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页码：997 / 1000
页数：4
相关论文
共 16 条
[1]  
BASELT RC, 1989, J ANAL TOXICOL, V13, P1
[2]   SYNTHETIC PEPTIDES AS ANTIGENS - PITFALLS OF CONJUGATION METHODS [J].
BRIAND, JP ;
MULLER, S ;
VANREGENMORTEL, MHV .
JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 78 (01) :59-69
[3]  
Bryant A.T., 1909, SOUTH AFR HUMANIT, V2, P1, DOI DOI 10.1097/00007611-190910000-00022
[4]   OCCURRENCE OF ATRACTYLOSIDE IN CALLILEPIS-LAUREOLA [J].
CANDY, HA ;
PEGEL, KH .
PHYTOCHEMISTRY, 1977, 16 (08) :1308-1309
[5]  
CONTESSA AR, 1978, ATRACTYLOSIDE CHEM B, P1
[6]  
FUCHS S, 1969, BIOCHIM BIOPHYS ACTA, V18, P384
[7]   PREPARATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO THE TRICHOTHECENE MYCOTOXIN T-2 [J].
HUNTER, KW ;
BRIMFIELD, AA ;
MILLER, M ;
FINKELMAN, FD ;
CHU, SF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 49 (01) :168-172
[8]  
Pegel K.H., 1981, CHEM ENG NEWS, V59, P4
[9]   THEORY FOR DISPLACEMENT OF PROTEINS AND VIRUSES WITH POLYETHYLENE-GLYCOL [J].
POLSON, A .
PREPARATIVE BIOCHEMISTRY, 1977, 7 (02) :129-154
[10]   NEW ATRACTYLIGENINE GLYCOSIDE FROM GREEN COFFEE-BEANS [J].
RICHTER, H ;
SPITELLER, G .
CHEMISCHE BERICHTE-RECUEIL, 1978, 111 (10) :3506-3509
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