ENVIRONMENTALLY COMPATIBLE SYNTHESIS OF CATECHOL FROM D-GLUCOSE

被引：52

作者：

DRATHS, KM ^{[1
]}

FROST, JW ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV, DEPT CHEM, E LANSING, MI 48824 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1995年 / 117卷 / 09期

关键词：

D O I：

10.1021/ja00114a003

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A biocatalytic alternative to currently employed industrial synthesis of catechol has been developed. Klebsiella pneumoniae genes encoding 3-dehydroshikimate dehydratase (aroZ) and protocatechuic acid decarboxylase (aroY) were introduced into an Escherichia coli construct that synthesizes elevated levels of 3-dehydroshikimic acid. One of the resulting biocatalysts, E. coli AB2834/pKD136/pKD9.069A, synthesizes 18.5 +/- 2.0 mM catechol from 56 mM D-glucose on 1 L scale.

引用

页码：2395 / 2400

页数：6

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