MONITORING OF ETHANOL DURING FERMENTATION OF A LIGNOCELLULOSE HYDROLYSATE BY ONLINE MICRODIALYSIS SAMPLING, COLUMN LIQUID-CHROMATOGRAPHY, AND AN ALCOHOL BIOSENSOR

被引：38

作者：

BUTTLER, T ^{[1
]}

GORTON, L ^{[1
]}

JARSKOG, H ^{[1
]}

MARKOVARGA, G ^{[1
]}

HAHNHAGERDAL, B ^{[1
]}

MEINANDER, N ^{[1
]}

OLSSON, L ^{[1
]}

机构：

[1] LUND UNIV,DEPT APPL MICROBIOL,LUND,SWEDEN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1994年 / 44卷 / 03期

关键词：

BIOSENSOR; ETHANOL; FERMENTATION; LIGNOCELLULOSE HYDROLYSATE; LIQUID CHROMATOGRAPHY; MICRODIALYSIS; ONLINE; SAMPLING;

D O I：

10.1002/bit.260440309

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

During a 70-h fermentation of a lignocellulose hydrolysate, the ethanol produced was monitored on-line using a microdialysis probe as an in situ sampling device. The dialysate components were then separated in a column liquid chromatographic system and the ethanol was selectively detected by an amperometric alcohol biosensor. The result was compared with two off-line analysis methods: one chromatographic method with refractive index (RI) detection and one enzymatic method based on spectrophotometric detection. The two methods based on enzymes were shown to give lower values than the chromatographic method based on RI detection, which is discussed in terms of selectivity. The investigated on-line setup was found to be a flexible system for monitoring of fermentations, allowing a sampling frequency of at least 12 h(-1) and with a delay between sampling and detection of less than 5 min. (C) 1994 John Wiley & Sons, Inc.

引用

页码：322 / 328

页数：7

共 14 条

[1] ONLINE FERMENTATION PROCESS MONITORING OF CARBOHYDRATES AND ETHANOL USING TANGENTIAL FLOW FILTRATION AND COLUMN LIQUID-CHROMATOGRAPHY [J].