AN ISOFORM OF THE HUMAN CALCITONIN RECEPTOR IS EXPRESSED IN TT CELLS AND IN MEDULLARY CARCINOMA OF THE THYROID

被引：37

作者：

FRENDO, JL ^{[1
]}

PICHAUD, F ^{[1
]}

MOURROUX, RD ^{[1
]}

BOUIZAR, Z ^{[1
]}

SEGOND, N ^{[1
]}

MOUKHTAR, MS ^{[1
]}

JULLIENNE, A ^{[1
]}

机构：

[1] CTR VIGGO PETERSEN, INSERM, U349, F-75010 PARIS, FRANCE

来源：

FEBS LETTERS | 1994年 / 342卷 / 02期

关键词：

RNA; EXPRESSION; TRUNCATED; AUTOCRINE; PARACRINE; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0014-5793(94)80503-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained band that hybridized to a CTR probe. Sequencing the band amplified from TT cells revealed an open reading frame identical to the sequence of H-CTR but lacking 16 amino acids in the first intracellular loop. This demonstrates the existence of an mRNA coding for a subtype of H-CTR which is expressed in TT cells and MTC.

引用

页码：214 / 216

页数：3

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