A NEW CONTINUOUS OPTICAL ASSAY FOR MALTASE AND SUCRASE

被引:3
|
作者
GIORGI, M [1 ]
VANNI, P [1 ]
PINZAUTI, G [1 ]
机构
[1] DIPARTIMENTO SCI BIOCHIM,VIALE MORGAGNI 50,I-50134 FLORENCE,ITALY
关键词
MALTASE; NEUTRAL; ENZYME ASSAYS; ALPHA-GLUCOSIDASES;
D O I
10.1159/000468807
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new method for the assay of maltase and sucrase is reported. The method makes use of mutarotase, hexokinase and glucose 6-phosphate dehydrogenase as ancillary enzymes. The reaction is linear at least up to a DELTAE/min of 0.13.
引用
收藏
页码:299 / 303
页数:5
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