CHANGES OF PHORBOL ESTER BINDING-SITES IN RAT-BRAIN FOLLOWING INTRACEREBROVENTRICULAR ADMINISTRATION OF THYROTROPIN-RELEASING-HORMONE (TRH) - AN INVITRO MACROAUTORADIOGRAPHIC INVESTIGATION

被引：5

作者：

MIZUKAWA, K ^{[1
]}

OTSUKA, N ^{[1
]}

OGAWA, N ^{[1
]}

HABA, K ^{[1
]}

MORI, A ^{[1
]}

机构：

[1] OKAYAMA UNIV,SCH MED,DEPT NEUROBIOL,OKAYAMA 700,JAPAN

来源：

NEUROCHEMICAL RESEARCH | 1992年 / 17卷 / 04期

关键词：

TRH; PHORBOL ESTER; PROTEIN KINASE-C; INVITRO MACROAUTORADIOGRAPHY;

D O I：

10.1007/BF00974578

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the influence of the intracerebroventricular (icv) administration of thyrotropin-releasing hormone (TRH) on protein kinase C (PKC) activities in various rat forebrain regions in order to cast light on the mechanism of extra-pituitary non-endocrine physiological actions of TRH in the central nervous system. An in vitro macroautoradiographic method, with [H-3]phorbol 12, 13-dibutyrate (PDBu) as the radioactive ligand, was used to investigate quantitative alterations of PKC activities. The optical densities for PDBu binding sites in the striatum and hippocampal formation were significantly increased after the icv administration of TRH, while those in the frontal cortex and septum were unchanged. These findings suggest that TRH may exert some of its non-endocrine functions through striatal and hippocampal neurons which used PKC in their second messenger systems.

引用

页码：361 / 365

页数：5