COMPARISON BETWEEN ELISA AND BIOTIN-LABELED PROBES FROM CLONED CDNA OF POTATO VIRUS-X FOR THE DETECTION OF VIRUS IN CRUDE TUBER EXTRACTS

被引：7
作者：
EWEIDA, M [1 ]
XU, H [1 ]
SINGH, RP [1 ]
ABOUHAIDAR, MG [1 ]
机构：
[1] AGR CANADA,RES STN,RES BRANCH,FREDERICTON E3B 4Z7,NB,CANADA
来源：
PLANT PATHOLOGY | 1990年 / 39卷 / 04期
关键词：
D O I：
10.1111/j.1365-3059.1990.tb02544.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Diseases caused by potato virus X (PVX) are of significant agronomic importance, and early detection is vital. Biotinylated DNA probes were prepared by random‐primed labelling from cDNA inserts and used for the detection of PVX in crude extracts of infected tubers. The minimum detection level in these extracts is in the order of femtograms of PVX RNA. Comparison with ELISA showed that the hybridization method is 100–250 times more sensitive. Probes prepared by this method are highly specific for target RNA even in crude tuber extracts. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码：623 / 628
页数：6
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