IMMOBILIZATION OF ASPERGILLUS-NIGER XYLANASE ON MAGNETIC LATEX BEADS

被引：32
|
作者：
TYAGI, R
GUPTA, MN
机构：
[1] Chemistry Department, Indian Institute of Technology, Delhi
来源：
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 1995年 / 21卷
关键词：
D O I：
10.1111/j.1470-8744.1995.tb00332.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Xylanase from Pectinex 3XL was purified 70-fold by precipitation with an enteric polymer, Eudragit S-100. The purified xylanase was immobilized on magnetic latex beads via carbodi-imide coupling. The immobilized preparation showed 80% of the total activity bound to the beads. The pH optimum remained unchanged at 6.0 and the K-m increased from 0.25 g/100 ml (free enzyme) to 0.39 g/100 mi on immobilization. Immobilization resulted in significant thermal stability at 60 degrees C. The time course of hydrolysis of xylan at 60 degrees C by free enzyme as well as immobilized enzyme was also studied.
引用
收藏
页码：217 / 222
页数：6
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