CAPILLARY ZONE ELECTROPHORESIS IN POLYMER NETWORKS OF POLYMERASE CHAIN REACTION-AMPLIFIED OLIGONUCLEOTIDES - THE CASE OF CONGENITAL ADRENAL-HYPERPLASIA

被引：20
作者：
GELFI, C
ORSI, A
RIGHETTI, PG
ZANUSSI, M
CARRERA, P
FERRARI, M
机构：
[1] UNIV MILAN,FAC PHARM,MILAN,ITALY
[2] CNR,ITBA,I-20133 MILAN,ITALY
[3] UNIV MILAN,DEPT BIOMED SCI & TECHNOL,I-20133 MILAN,ITALY
[4] IST SCI HS RAFFAELE,CENT LAB,MILAN SEGRATE,ITALY
[5] IST SCI HS RAFFAELE,DIBIT,MILAN SEGRATE,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 657卷 / 01期
关键词：
D O I：
10.1016/0378-4347(94)80088-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 mu M ethidium bromide for improving DNA fragment separation, Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for >50 analyses before degradation and loss of resolution. The results are comparable to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining.
引用
收藏
页码：201 / 205
页数：5
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