CAPILLARY ZONE ELECTROPHORESIS IN POLYMER NETWORKS OF POLYMERASE CHAIN REACTION-AMPLIFIED OLIGONUCLEOTIDES - THE CASE OF CONGENITAL ADRENAL-HYPERPLASIA

The use of capillary zone electrophoresis (CZE) in polymer networks for the analysis of an 8 bp (base pair) deletion in congenital adrenal hyperplasia was investigated. Separations were performed in Tris-borate-EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 mu M ethidium bromide for improving DNA fragment separation, Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be used for >50 analyses before degradation and loss of resolution. The results are comparable to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV absorption at 254 nm, is similar to that obtained in gel slabs by dye intercalation staining.