A NOVEL MYOSIN HEAVY-CHAIN ISOFORM IN VASCULAR SMOOTH-MUSCLE

被引：11

作者：

OKAIMATSUO, Y

TAKANOOHMURO, H

TOYOOKA, T

SUGIMOTO, T

机构：

[1] UNIV TOKYO,CTR HLTH SERV,DEPT INTERNAL MED 2,TOKYO 113,JAPAN

[2] TOKYO METROPOLITAN INST MED SCI,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90437-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies demonstrated two myosin heavy chain isoforms in vascular smooth muscles with SDS-polyacrylamide gel electrophoresis; MHC1 (204kDa) and MHC2 (200kDa). We report the existence of a novel myosin heavy chain isoform, MHC3 (196kDa), which was exclusively contained in inferior vena cava. Equal amount of MHC1 and MHC2 was observed in aorta and pulmonary artery, respectively. However, inferior vena cava contained only MHC3. Proteolytic artifact was refuted by immunoblotting of tissue homogenates without purification, or SDS-polyacrylamide gel electrophoresis of myosin bands isolated by pyrophosphate gel electrophoresis. Furthermore, α-chymotryptic cleavage of MHC1, MHC2, and MHC3 displayed different peptide maps, indicating the primary structural difference among all three isoforms. © 1991.

引用

页码：1365 / 1370

页数：6

共 23 条

[1] ISOFORMS OF MYOSIN AND ACTIN IN HUMAN, MONKEY AND RAT MYOMETRIUM - COMPARISON OF PREGNANT AND NONPREGNANT UTERUS PROTEINS [J].