ANALYSIS OF CARBOHYDRATES IN MARINE PARTICULATES BY GAS-CHROMATOGRAPHY AND TANDEM MASS-SPECTROMETRY

被引：5

作者：

LESKOVSEK, H ^{[1
]}

PERKO, S ^{[1
]}

ZIGON, D ^{[1
]}

FAGANELI, J ^{[1
]}

机构：

[1] MARINE BIOL STN,66330 PIRAN,SLOVENIA

来源：

ANALYST | 1994年 / 119卷 / 06期

关键词：

MONOSACCHARIDE; ALDITOL ACETATE; GAS CHROMATOGRAPHY MASS SPECTROMETRY; GAS CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; MARINE SEDIMENTED PARTICULATE;

D O I：

10.1039/an9941901125

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of a derivatization procedure for the measurement of aldoses by forming alditol acetates by strong acid hydrolysis is described. Quantitative data based on standard mixtures of neutral sugars were obtained by capillary gas chromatography and gas chromatography-mass spectrometry using phenyl-B-D-glucopyranoside as an internal standard. Tandem mass spectrometry was used for the structural elucidation of complex saccharide mixtures. In order to differentiate between alditol acetates, i.e., hexitols, pentitols and deoxyhexitols, the collision-activated reactions m/z 361-->259, 303-->201 and 217-->115 were monitored by the Q sector of a VG-AutospecQ mass spectrometer. The method was applied to the study of the composition of polysaccharides in particulate organic matter and macroaggregates in the northern Adriatic Sea.

引用

页码：1125 / 1128

页数：4

共 9 条

[1]

Busch KL., 1988, MASS SPECTROMETRY MA

[2] DETERMINATION OF NEUTRAL SUGARS IN PLANKTON, SEDIMENTS, AND WOOD BY CAPILLARY GAS-CHROMATOGRAPHY OF EQUILIBRATED ISOMERIC MIXTURES [J].