HIERARCHICAL AND COOPERATIVE BINDING OF THE RAT-LIVER NUCLEAR-PROTEIN C/EBP AT THE HEPATITIS-B VIRUS ENHANCER

被引：42

作者：

DIKSTEIN, R ^{[1
]}

FAKTOR, O ^{[1
]}

SHAUL, Y ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT MOLEC GENET & VIROL,IL-76100 REHOVOT,ISRAEL

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 08期

关键词：

D O I：

10.1128/MCB.10.8.4427

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used the enhancer-binding protein C/EBP as a model to study the nature and the complexity of interaction of an enhancer-binding protein with its target DNA. We found that bacterially expressed C/EBP binds the hepatitis B virus enhancer at multiple sites in a hierarchic and cooperative manner. At low concentrations, only the E element is occupied, but at higher concentrations, additional sites are filled including a site that binds EP, a crucial enhancer-activating protein. This pattern of C/EBP binding may explain the concentration-dependent effect of C/EBP on enhancer activity.

引用

页码：4427 / 4430

页数：4

共 16 条

[1] CELLULAR FACTORS THAT INTERACT WITH THE HEPATITIS-B VIRUS ENHANCER [J].

BENLEVY, R ;

FAKTOR, O ;

BERGER, I ;

SHAUL, Y .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (04) :1804-1809

[2] TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN [J].

BIRKENMEIER, EH ;

GWYNN, B ;

HOWARD, S ;

JERRY, J ;

GORDON, JI ;

LANDSCHULZ, WH ;

MCKNIGHT, SL .

GENES & DEVELOPMENT, 1989, 3 (08) :1146-1156

[3] DIFFERENTIATION-INDUCED GENE-EXPRESSION IN 3T3-L1 PREADIPOCYTES - CCAAT ENHANCER BINDING-PROTEIN INTERACTS WITH AND ACTIVATES THE PROMOTERS OF 2 ADIPOCYTE-SPECIFIC GENES [J].

CHRISTY, RJ ;

YANG, VW ;

NTAMBI, JM ;

GEIMAN, DE ;

LANDSCHULZ, WH ;

FRIEDMAN, AD ;

NAKABEPPU, Y ;

KELLY, TJ ;

LANE, MD .

GENES & DEVELOPMENT, 1989, 3 (09) :1323-1335

[4] FUNCTIONAL-ORGANIZATION OF THE HEPATITIS-B VIRUS ENHANCER [J].

DIKSTEIN, R ;

FAKTOR, O ;

BENLEVY, R ;

SHAUL, Y .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (07) :3683-3689

[5] A SINGLE ELEMENT WITHIN THE HEPATITIS-B VIRUS ENHANCER BINDS MULTIPLE PROTEINS AND RESPONDS TO MULTIPLE STIMULI [J].

FAKTOR, O ;

BUDLOVSKY, S ;

BENLEVY, R ;

SHAUL, Y .

JOURNAL OF VIROLOGY, 1990, 64 (04) :1861-1863

[6] CCAAT ENHANCER BINDING-PROTEIN ACTIVATES THE PROMOTER OF THE SERUM-ALBUMIN GENE IN CULTURED HEPATOMA-CELLS [J].

FRIEDMAN, AD ;

LANDSCHULZ, WH ;

MCKNIGHT, SL .

GENES & DEVELOPMENT, 1989, 3 (09) :1314-1322

[7] DNAASE FOOTPRINTING - SIMPLE METHOD FOR DETECTION OF PROTEIN-DNA BINDING SPECIFICITY [J].

GALAS, DJ ;

SCHMITZ, A .

NUCLEIC ACIDS RESEARCH, 1978, 5 (09) :3157-3170

[8] LIVER-SPECIFIC EXPRESSION OF HEPATITIS-B VIRUS IS DETERMINED BY THE COMBINED ACTION OF THE CORE GENE PROMOTER AND THE ENHANCER [J].

HONIGWACHS, J ;

FAKTOR, O ;

DIKSTEIN, R ;

SHAUL, Y ;

LAUB, O .

JOURNAL OF VIROLOGY, 1989, 63 (02) :919-924

[9] THE HUMAN HEPATITIS-B VIRUS ENHANCER REQUIRES TRANS-ACTING CELLULAR FACTOR(S) FOR ACTIVITY [J].

JAMEEL, S ;

SIDDIQUI, A .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (02) :710-715

[10] IDENTIFICATION OF A RAT-LIVER NUCLEAR-PROTEIN THAT BINDS TO THE ENHANCER CORE ELEMENT OF 3 ANIMAL VIRUSES [J].

JOHNSON, PF ;

LANDSCHULZ, WH ;

GRAVES, BJ ;

MCKNIGHT, SL .

GENES & DEVELOPMENT, 1987, 1 (02) :133-146

← 1 2 →