Multilocus genotyping of Giardia duodenalis infecting rabbits in Ogun State, Nigeria

Giardiasis is a cosmopolitan gastrointestinal protozoal parasite that infects humans and various animals worldwide. To assess the zoonotic transmission potential of Giardia, molecular characterization is required. We are unaware of any report on the genotypes of Giardia infecting rabbits in Nigeria. Molecular detection and genotyping of Giardia duodenalis were conducted in a herd of adult Chinchilla rabbits (Oryctolagus cuniculus) managed on the Teaching and Research farm of the Federal University of Agriculture, Abeokuta located in a southwestern state of Nigeria by analysis of the small-subunit ribosomal RNA (ssu rRNA), glutamate dehydrogenase (gdh), triosephosphate isomerase (tpi) and beta-giardin (bg) genes. An overall prevalence of 72.3% (60/83) was recorded in the rabbits with no statistically significant (p > .05) influence of sex on the distribution of the infection in the herd. All the 19 isolates amplified at the four genetic loci were identified as G. duodenalis assemblage BIV by multiple alignment analysis of their consensus sequences. Novel nucleotide substitutions were identified in two isolates at the ssu rRNA locus. Phylogenetic analysis revealed that all ssu rRNA genotypes were closely related to G. duodenalis assemblage B of cattle and human origin. Findings of this study suggest that the rabbits harbour potentially zoonotic assemblage BIV that portends a high risk to students and staff of the University who are in regular contact with the animals.