ADP-RIBOSYLATION OF RHO ENHANCES ACTIN POLYMERIZATION-COUPLED SHAPE OSCILLATIONS IN HUMAN NEUTROPHILS

被引：21
作者：
EHRENGRUBER, MU
BOQUET, P
COATES, TD
DERANLEAU, DA
机构：
[1] UNIV SO CALIF,CHILDRENS HOSP LOS ANGELES,DIV HEMATOL ONCOL,LOS ANGELES,CA 90027
[2] UNIV BERN,THEODOR KOCHER INST,CH-3012 BERN,SWITZERLAND
[3] INST PASTEUR,DEPT BACTERIOL,UNITE TOXINES MICROBIENNES,CNRS,URA 557,F-75724 PARIS 15,FRANCE
来源：
FEBS LETTERS | 1995年 / 372卷 / 2-3期
关键词：
CYTOSKELETON; POLYMORPHONUCLEAR LEUKOCYTE; TURBIDIMETRY; G PROTEIN; EXOENZYME C3; WORTMANNIN;
D O I：
10.1016/0014-5793(95)00880-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Stimulated neutrophils exhibit coordinated sinusoidal oscillations in filamentous actin content and cellular shape. We investigated the effect of inhibition of the small G protein Rho on neutrophil actin polymerization, shape changes and oscillations using a genetically engineered toxin that enters cells and selectively ADP-ribosylates endogenous Rho. This treatment increased the amplitudes and frequencies of shape oscillations and duration of the oscillating transient. However, it had no effect on the initial actin polymerization and shape changes induced by N-formyl-Met-Leu-Phe. Regulation of these oscillations may be important for the control of neutrophil motility.
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收藏
页码：161 / 164
页数：4
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