STEROL 27-HYDROXYLASE - HIGH-LEVELS OF ACTIVITY IN VASCULAR ENDOTHELIUM

被引：0

作者：

REISS, AB ^{[1
]}

MARTIN, KO ^{[1
]}

JAVITT, NB ^{[1
]}

MARTIN, DW ^{[1
]}

GROSSI, EA ^{[1
]}

GALLOWAY, AC ^{[1
]}

机构：

[1] NYU,MED CTR,DEPT SURG,NEW YORK,NY 10016

来源：

JOURNAL OF LIPID RESEARCH | 1994年 / 35卷 / 06期

关键词：

BOVINE AORTIC ENDOTHELIAL CELLS; HEPG2; CELLS; CHO CELLS; 27-HYDROXYCHOLESTEROL; 3-BETA-HYDROXY-5-CHOLESTENOIC ACID;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sterol 27-hydroxylase activity in bovine aortic endothelial (BAE) cells in culture has been compared with that in HepG2 cells and in Chinese hamster ovary (CHO) cells using identical culture conditions. The total enzyme activity of BAE cells (3.0 nmol/72 h per mg cell protein) was comparable with that of HepG2 cells (4.0 nmol/72 h per mg protein) and both values were significantly greater than that in CHO cells (0.002 nmol/72 h per mg protein). The enzyme was identified in the mitochondria extracted from BAE cells by Western blotting using an antibody of proven specificity, and its metabolites 27-hydroxycholesterol and 3 beta-hydroxy-5-cholestenoic acid were identified by mass spectrum analysis. The presence of the enzyme in endothelium provides a mechanism for preventing accumulation of intracellular cholesterol by initiating a pathway of bile acid synthesis different from that initiated by 7 alpha-hydroxylation of cholesterol in the liver.

引用

页码：1026 / 1030

页数：5

共 22 条

[11] BILE-ACID SYNTHESIS IN MAN - INVIVO ACTIVITY OF THE 25-HYDROXYLATION PATHWAY [J].