ACTIVITY AND STABILITY OF YEAST ALCOHOL-DEHYDROGENASE (YADH) ENTRAPPED IN AEROSOL OT REVERSE MICELLES

被引：65
|
作者：
SARCAR, S [1 ]
JAIN, TK [1 ]
MAITRA, A [1 ]
机构：
[1] UNIV DELHI,DEPT CHEM,DELHI 110007,INDIA
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 04期
关键词：
YEAST ALCOHOL DEHYDROGENASE; AOT REVERSE MICELLES; ENZYME ACTIVITY; REVERSE MICELLES;
D O I：
10.1002/bit.260390416
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The activity and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micellar droplets have been investigated spectrophotometrically. Various physical parameters, e.g., water pool size, W0, pH, and temperature, were optimized for YADH in water/AOT/isooctane reverse micelles. It was found that the enzyme exhibits maximum activity at W0 = 28 and pH 8.1. It was more active in reverse micelles than in aqueous buffers at a particular temperature and was denatured at about 30-degrees-C in both the systems. At a particular temperature YADH entrapped in reverse micelles was less stable than when it was dissolved in aqueous buffer.
引用
收藏
页码：474 / 478
页数：5
相关论文
共 50 条
  • [31] SUBUNIT CONFORMATION OF YEAST ALCOHOL-DEHYDROGENASE
    JORNVALL, H
    EKLUND, H
    BRANDEN, CI
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1978, 253 (23) : 8414 - 8419
  • [32] Hydrophobized formate dehydrogenase from Pseudomonas sp 101 in the system of reverse micelles of aerosol OT in octane
    Trorimova, DN
    Levashov, AV
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2002, 28 (05) : 392 - 396
  • [33] PROTEIN DYNAMICAL STRUCTURE BY TRYPTOPHAN PHOSPHORESCENCE AND ENZYMATIC-ACTIVITY IN REVERSE MICELLES .1. LIVER ALCOHOL-DEHYDROGENASE
    STRAMBINI, GB
    GONNELLI, M
    JOURNAL OF PHYSICAL CHEMISTRY, 1988, 92 (10): : 2850 - 2853
  • [34] Molecular Dynamics Simulation of Aerosol-OT Reverse Micelles
    Chowdhary, Janamejaya
    Ladanyi, Branka M.
    JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (45): : 15029 - 15039
  • [35] KINETIC MECHANISM OF YEAST ALCOHOL-DEHYDROGENASE ACTIVITY WITH SECONDARY ALCOHOLS AND KETONES
    TRIVIC, S
    LESKOVAC, V
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 1994, 31 (05): : 387 - 391
  • [36] YEAST ALCOHOL-DEHYDROGENASE ACTIVITY ON SUBSTITUTED 3-PHENYL-PROPANOLS
    SUND, EH
    LOVE, RW
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1977, 173 (MAR20): : 48 - 48
  • [37] Functionalization of (CdSe)ZnS nanoparticles in reverse micelles of Aerosol OT
    Eremin, A. N.
    Zhavnerko, G. K.
    Agabekov, V. E.
    COLLOID JOURNAL, 2012, 74 (04) : 445 - 455
  • [38] Functionalization of (CdSe)ZnS nanoparticles in reverse micelles of Aerosol OT
    A. N. Eremin
    G. K. Zhavnerko
    V. E. Agabekov
    Colloid Journal, 2012, 74 : 445 - 455
  • [39] Photophysics of disulphonated aluminium phthalocyanine in reverse micelles of Aerosol OT
    Foley, MSC
    Beeby, A
    Parker, AW
    Bishop, SM
    Phillips, D
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1997, 38 (01) : 18 - 24
  • [40] Formose reaction accelerated in aerosol-OT reverse micelles
    Masaoka, Makoto
    Michitaka, Tomohiro
    Hashidzume, Akihito
    BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY, 2016, 12 : 2663 - 2667
12345