ACTIVITY AND STABILITY OF YEAST ALCOHOL-DEHYDROGENASE (YADH) ENTRAPPED IN AEROSOL OT REVERSE MICELLES

被引：66

作者：

SARCAR, S ^{[1
]}

JAIN, TK ^{[1
]}

MAITRA, A ^{[1
]}

机构：

[1] UNIV DELHI,DEPT CHEM,DELHI 110007,INDIA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 04期

关键词：

YEAST ALCOHOL DEHYDROGENASE; AOT REVERSE MICELLES; ENZYME ACTIVITY; REVERSE MICELLES;

D O I：

10.1002/bit.260390416

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activity and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micellar droplets have been investigated spectrophotometrically. Various physical parameters, e.g., water pool size, W0, pH, and temperature, were optimized for YADH in water/AOT/isooctane reverse micelles. It was found that the enzyme exhibits maximum activity at W0 = 28 and pH 8.1. It was more active in reverse micelles than in aqueous buffers at a particular temperature and was denatured at about 30-degrees-C in both the systems. At a particular temperature YADH entrapped in reverse micelles was less stable than when it was dissolved in aqueous buffer.

引用

页码：474 / 478

页数：5

共 39 条

[1] MICELLAR SOLUBILIZATION OF BIO-POLYMERS IN ORGANIC-SOLVENTS .5. ACTIVITY AND CONFORMATION OF ALPHA-CHYMOTRYPSIN IN ISOOCTANE-AOT REVERSE MICELLES [J].