Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots

被引：2

作者：

Xiu Jing-rui ^{[1
]}

Hu Si-yi ^{[1
]}

Li Jin-hua ^{[1
]}

Ren Sheng ^{[1
]}

Liu Li-wei ^{[2
]}

机构：

[1] Changchun Univ Sci & Technol, Coll Sci, Changchun 130022, Jilin, Peoples R China

[2] Shenzhen Univ, Coll Optoelect Engn, Shenzhen 518060, Peoples R China

来源：

CHINESE OPTICS | 2018年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

quantum dots; FRET; near infrared; pH sensitive;

D O I：

10.3788/CO.20181101.0074

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

In this paper, a kind of fluorescence resonance energy transfer( FRET) system based on near infrared InP/ZnS Quantum Dots and fluorescence dye Cy7 was constructed, and the conversion efficiencies of FRET system at different pH values and different concentrations were measured. Experimental results indicated that when the concentration of quanium dots remained constant, the conversion efficiency of the system increased with the increasing of the concentration of dye. When the concentration ratio of InP/ZnS and Cy7 was 1:250, the conversion efficiency was 68%. The results of cell test showed that the FRET system had a high sensitivity to pH value, and the detection accuracy of pH value for cell microenvironment was 0.1, which could be used as a sensitive FRET probe for biological microenvironment detection.

引用

页码：74 / 82

页数：9

共 23 条

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