GLUTATHIONE AS A MATRIX FOR PLASMA DESORPTION MASS-SPECTROMETRY OF LARGE PEPTIDES

被引：57

作者：

ALAI, M ^{[1
]}

DEMIREV, P ^{[1
]}

FENSELAU, C ^{[1
]}

COTTER, RJ ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,MIDDLE ATLANTIC MASS SPECTROMET FACIL,DEPT PHARMACOL,BALTIMORE,MD 21218

来源：

ANALYTICAL CHEMISTRY | 1986年 / 58卷 / 07期

关键词：

ORGANIC COMPOUNDS - Spectroscopic Analysis;

D O I：

10.1021/ac00298a008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The plasma desorption mass spectra of large peptides, dissolved and electrosprayed in solutions containing glutathione, showed increased molecular ion signal, reduction of base-line noise and peak widths, and an increase in multiply charged ions. The reduced, rather than the oxidized, form of glutathione is responsible for these effects. Some other chemically similar matrices showed similar effects while others do not. Several roles for the matrix are suggested including previously reported effects on protein refolding and aggregation in solution, as well as possibilities for lowering the sample/substrate binding energy during desorption.

引用

页码：1303 / 1307

页数：5

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