A SANDWICH ENZYME-IMMUNOASSAY FOR BOVINE INTERFERON-GAMMA AND ITS USE FOR THE DETECTION OF TUBERCULOSIS IN CATTLE

被引：276
作者：
ROTHEL, JS
JONES, SL
CORNER, LA
COX, JC
WOOD, PR
机构：
[1] CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria
来源：
AUSTRALIAN VETERINARY JOURNAL | 1990年 / 67卷 / 04期
关键词：
D O I：
10.1111/j.1751-0813.1990.tb07730.x
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
An in vitro cellular assay for bovine tuberculosis has recently been developed. This assay detects gamma-interferon released in response to specific antigen in a whole blood culture system. The bio-assay previously described for the detection of bovine gamma-interferon (IFN-gamma) has now been replaced with a sandwich enzyme immunoassay (EIA) which utilises two monoclonal antibodies to bovine IFN-gamma. The EIA detects less than 25pg/ml of recombinant bovine IFN-gamma and is specific for biologically active bovine IFN-gamma; and does not detect bovine alpha or beta interferon. IFN-gamma from sheep, goat and buffalo, but not from pig, deer or man, are also recognised by the EIA. The bovine IFN-gamma EIA when used in conjunction with the whole blood culture system has resulted in a simple, rapid and sensitive in vitro assay for specific cell mediated immune responsiveness to M. bovis infection in cattle.
引用
收藏
页码：134 / 137
页数：4
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