DETECTION OF BOVINE VIRUS DIARRHEA VIRUS-RNA BY IN-SITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED RIBOPROBES

被引：0
|
作者：
DESPORT, M
COLLINS, ME
BROWNLIE, J
机构：
来源：
INTERVIROLOGY | 1994年 / 37卷 / 05期
关键词：
BOVINE VIRUS DIARRHEA VIRUS; IN SITU HYBRIDIZATION; DIGOXIGENIN; IMMUNOCYTOCHEMISTRY; PESTIVIRUS;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A non-isotopic in situ hybridisation (ISH) method has been developed for the detection of bovine virus diarrhoea virus (BVDV) RNA. The technique is highly specific and allows the detection of BVDV in formalin-fixed paraffin-wax-embedded sections. Digoxigenin-labelled riboprobes were generated to the coding region for the p125 non-structural protein and consequently it was possible to locate the virus positive-sense RNA in BVDV-infected tissues. Formalin-fixed tissues are ideal for ISH, combining the possibility of a retrospective analysis of tissues for virus distribution with excellent morphological preservation. In situ hybridisation has facilitated a precise examination, at the cellular level, of viral nucleic acid distribution during pestiviral infections, with an increased sensitivity and wider applicability than immunocytochemistry.
引用
收藏
页码：269 / 276
页数：8
相关论文
共 50 条
  • [41] VIRUS-RELATED SEROLOGY AND IN-SITU HYBRIDIZATION FOR THE DETECTION OF VIRUS-DNA AMONG PATIENTS WITH TESTICULAR CANCER
    HEINZER, H
    DIECKMANN, KP
    HULAND, E
    EUROPEAN UROLOGY, 1993, 24 (02) : 271 - 276
  • [42] Bovine respiratory syncytial virus in-situ hybridization from sheep lungs at different times postinfection
    Redondo, E
    Gomez, L
    Kelling, CL
    Gazquez, A
    Masot, AJ
    ARCHIVOS DE MEDICINA VETERINARIA, 2003, 35 (01) : 37 - 49
  • [43] FLOW CYTOMETRIC DETECTION OF BOVINE VIRAL DIARRHEA VIRUS
    QVIST, P
    HOUE, H
    AASTED, B
    MEYLING, A
    RONSHOLT, L
    BLOCH, B
    ARCHIVES OF VIROLOGY, 1991, : 165 - 167
  • [44] In situ detection of reovirus in formalin-fixed, paraffin-embedded chicken tissues using a digoxigenin-labeled cDNA probe
    Liu, HJ
    Giambrone, JJ
    AVIAN DISEASES, 1997, 41 (02) : 447 - 451
  • [45] In-situ Hybridization for the Detection of Sacbrood Virus in Infected Larvae of the Honey Bee (Apis cerana)
    Park, C.
    Kang, H. S.
    Jeong, J.
    Kang, I.
    Choi, K.
    Yoo, M. -S.
    Kim, Y. -H.
    Kang, S. -W.
    Lim, H. -Y.
    Yoon, B. -S.
    Chae, C.
    JOURNAL OF COMPARATIVE PATHOLOGY, 2016, 154 (2-3) : 258 - 262
  • [46] Production of digoxigenin-labelled RNA probes and the detection of cytokine mRNA in rat spleen and brain by in situ hybridization
    Meltzer, JC
    Sanders, V
    Grimm, PC
    Stern, E
    Rivier, C
    Lee, S
    Rennie, SL
    Gietz, RD
    Hole, AK
    Watson, PH
    Greenberg, AH
    Nance, DM
    BRAIN RESEARCH PROTOCOLS, 1998, 2 (04): : 339 - 351
  • [47] Detection of bovine viral diarrhea virus and bovine herpes virus type 1 in cattle with and without endometritis
    Atasever, Ayhan
    Mendil, Ali Sefa
    Timurkan, Mehmet Ozkan
    VETERINARY RESEARCH FORUM, 2023, 14 (10) : 541 - 547
  • [48] RNA In Situ Hybridization for Epstein-Barr Virus and Cytomegalovirus: Comparison With In Situ Hybridization and Immunohistochemistry
    Roe, Catherine J.
    Siddiqui, Momin T.
    Lawson, Diane
    Cohen, Cynthia
    APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 2019, 27 (02) : 155 - 159
  • [49] EPSTEIN-BARR-VIRUS DETECTION BY IN-SITU HYBRIDIZATION IN FINE-NEEDLE ASPIRATION BIOPSIES
    PACCHIONI, D
    NEGRO, F
    VALENTE, G
    BUSSOLATI, G
    DIAGNOSTIC MOLECULAR PATHOLOGY, 1994, 3 (02) : 100 - 104
  • [50] Demonstration of apoptotic cells in tissue sections by in situ hybridization using digoxigenin-labeled poly(A) oligonucleotide probes to detect thymidine-rich DNA sequences
    Hilton, DA
    Love, S
    Barber, R
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1997, 45 (01) : 13 - 20
12345