DETECTION OF BOVINE VIRUS DIARRHEA VIRUS-RNA BY IN-SITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED RIBOPROBES

被引：0
|
作者：
DESPORT, M
COLLINS, ME
BROWNLIE, J
机构：
来源：
INTERVIROLOGY | 1994年 / 37卷 / 05期
关键词：
BOVINE VIRUS DIARRHEA VIRUS; IN SITU HYBRIDIZATION; DIGOXIGENIN; IMMUNOCYTOCHEMISTRY; PESTIVIRUS;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A non-isotopic in situ hybridisation (ISH) method has been developed for the detection of bovine virus diarrhoea virus (BVDV) RNA. The technique is highly specific and allows the detection of BVDV in formalin-fixed paraffin-wax-embedded sections. Digoxigenin-labelled riboprobes were generated to the coding region for the p125 non-structural protein and consequently it was possible to locate the virus positive-sense RNA in BVDV-infected tissues. Formalin-fixed tissues are ideal for ISH, combining the possibility of a retrospective analysis of tissues for virus distribution with excellent morphological preservation. In situ hybridisation has facilitated a precise examination, at the cellular level, of viral nucleic acid distribution during pestiviral infections, with an increased sensitivity and wider applicability than immunocytochemistry.
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页码：269 / 276
页数：8
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