VARIATION OF LYSOZYME SOLUBILITY AS A FUNCTION OF TEMPERATURE IN THE PRESENCE OF ORGANIC AND INORGANIC SALTS

被引：85
作者：
GUILLOTEAU, JP
RIESKAUTT, MM
DUCRUIX, AF
机构：
[1] Laboratoire de Cristallochimie, Institut de Chimie des Substances Naturelles, CNRS
来源：
JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期
关键词：
D O I：
10.1016/0022-0248(92)90249-I
中图分类号：
O7 [晶体学];
学科分类号：
0702 ; 070205 ; 0703 ; 080501 ;
摘要：
The regularity of salts in affecting lysozyme solubility (and other proteins having a basic pI) at pH 4.5 according to the lyotropic series has been completed by solubility curve measurements. The measurement of lysozyme/NaSCN solubility shows very small difference with lysozyme/KSCN solubility, confirming the slight influence of the nature of the cation. The solubility of lysozyme nitrate inserts between the values of lysozyme chloride and thiocyanate. Some organic salts have been tested as new crystallizing agents and the solubility curve of lysozyme in the presence of sodium para-toluenesulfonate bas been determined. The solubility and the crystal form of lysozyme is affected differently by temperature changes (18 and 40-degrees-C), depending on the nature of the crystallizing agent.
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页码：223 / 230
页数：8
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