EVALUATION OF SOLUTE HYDROPHOBICITY BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING AQUEOUS BINARY MOBILE PHASES

被引：29
作者：
KAIBARA, A [1 ]
HOHDA, C [1 ]
HIRATA, N [1 ]
HIROSE, M [1 ]
NAKAGAWA, T [1 ]
机构：
[1] KYOTO UNIV,FAC PHARMACEUT SCI,KYOTO 606,JAPAN
来源：
CHROMATOGRAPHIA | 1990年 / 29卷 / 5-6期
关键词：
Capacity factor variation; Column liquid chromatography; Displacement model; Hydrophobic parameter; Organic modifier concentration;
D O I：
10.1007/BF02317918
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The dependence of the capacity factor (k′) on the concentration of the organic modifier (D) in the aqueous binary mobile phase in reversed-phase high-performance liquid chromatography has been investigated to evaluate the hydrophobicity of the solute molecule. The r-values, defined as the slope of log k′ vs. log(1/D) plots, were measured for various solutes and related to the non-polar surface area and the partition coefficients. The r-value was found to be a good indication of solute hydrophobicity. Detailed investigation of the results allowed to consider statistically the molecular posture of the solute adsorbed onto the stationary alkyl ligand. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
引用
收藏
页码：275 / 288
页数：14
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