SHOTGUN CONCATENATION OF SYNTHETIC GENES - CONSTRUCTION OF CONCATEMERIC HUMAN CALCITONIN GENES

被引：5

作者：

IVANOV, IG

机构：

[1] Institute of Molecular Biology, Bulgarian Academy of Sciences

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 189卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90110-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new fast and reliable approach for construction of concatemeric synthetic genes is described. The method consists of shotgun polymerization of three truncated monomeric gene units using a specific linker, followed by cloning of the recombinant clones and screening them for the presence of concatemeric genes of defined length. The method is illustrated by constructing a series of tandemly repeated human calcitonin (hCT) genes. © 1990.

引用

页码：213 / 216

页数：4

共 10 条

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