THE LIM FAMILY TRANSCRIPTION FACTOR-ISL-1 REQUIRES CAMP RESPONSE ELEMENT BINDING-PROTEIN TO PROMOTE SOMATOSTATIN EXPRESSION IN PANCREATIC-ISLET CELLS

被引：111

作者：

LEONARD, J ^{[1
]}

SERUP, P ^{[1
]}

GONZALEZ, G ^{[1
]}

EDLUND, T ^{[1
]}

MONTMINY, M ^{[1
]}

机构：

[1] UMEA UNIV,DEPT MICROBIOL,S-90187 UMEA,SWEDEN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 14期

关键词：

CAMP RESPONSE ELEMENT; CELL SPECIFICITY; PHOSPHORYLATION INDEPENDENCE;

D O I：

10.1073/pnas.89.14.6247

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many eukaryotic genes are regulated by cAMP through a conserved cAMP response element (CRE). Here we show that, in the pancreatic islet cell line Tu6, a well-characterized CRE in the somatostatin gene does not provide cAMP responsiveness but functions as an essential element for its basal activity. DNA-binding and functional analyses indicate that the cAMP-responsive factor CREB regulates somatostatin expression in these cells without requirement for phosphorylation at the protein kinase A-regulated Ser-133 phosphorylation site. In addition to the CRE site, cell-specific expression of the somatostatin gene requires a second promoter element, which binds the recently characterized LIM family protein Isl-1. Thus, Isl-1 and CREB appear to synergize on the somatostatin promoter to stimulate high-level expression in Tu6 cells. The ability of CREB to function in a phosphorylation-independent manner suggests a mechanism by which this protein can regulate gene transcription.

引用

页码：6247 / 6251

页数：5

共 18 条

[1] THE PITUITARY-SPECIFIC TRANSCRIPTION FACTOR-GHF-1 IS A HOMEOBOX-CONTAINING PROTEIN [J].