A PROTEIN-KINASE SUBSTRATE IDENTIFIED BY THE 2-HYBRID SYSTEM

被引：184

作者：

YANG, XO

HUBBARD, EJA

CARLSON, M

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG,DEPT GENET & DEV,NEW YORK,NY 10032

[2] COLUMBIA UNIV COLL PHYS & SURG,INST CANC RES,NEW YORK,NY 10032

来源：

SCIENCE | 1992年 / 257卷 / 5070期

关键词：

D O I：

10.1126/science.1496382

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A genetic method, the two-hybrid system, was used to identify four genes encoding proteins that interact with the SNF1 protein kinase from yeast. One of the genes, SIP1, was independently isolated as a multicopy suppressor of defects caused by reduced SNF1 kinase activity, and genetic evidence supports its function in the SNF1 pathyway. The SIP1 protein co-immunoprecipitated with SNF1 and was phosphorylated in vitro. Thus, the two-hybrid system, which is applicable to any cloned gene, can be used to detect physical interactions between protein kinases and functionally related substrate proteins.

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页码：680 / 682

页数：3

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