PREVALENCE AND ACTIVATION PHENOTYPE OF SENDAI VIRUS-SPECIFIC CD4(+) T-CELLS

被引:31
作者
EWING, C [1 ]
TOPHAM, DJ [1 ]
DOHERTY, PC [1 ]
机构
[1] ST JUDE CHILDRENS RES HOSP, DEPT IMMUNOL, MEMPHIS, TN 38105 USA
关键词
D O I
10.1006/viro.1995.1329
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The relative prevalence of Sendai virus-specific CD4(+) T helper precursors (Thp) has been assessed by limiting dilution analysis (LDA). Within 10 days after intranasal inoculation of C57BL/6 mice, Thp prevalence increased from <1 in 10(5) to approximately 1 in 200 in CD4(+) T-cell-enriched spleen and mediastinal lymph node populations. These frequencies remained elevated relative to naive animals, with the majority of the Thp recovered at 2-3 months after infection being found in the spleen. The ''memory'' Thp express an ''activated'' L-selectin-low, CD44-high, alpha 4-integrin-high phenotype, comparable to that described previously for CD8(+) cytotoxic T lymphocyte precursors (CTLp) specific for Sendai virus. Background effects for the IL-2-based Thp analysis are, however, less predictable than those round previously for the Cr-51 release CTLp LDA, as the extent of non-virus-specific lymphokine production varies. However, provided the analysis is appropriately controlled, the approach does allow useful comparisons between phenotypically different subsets of antigen-specific CD4(+) T cells. (C) 1995 Academic Press, Inc.
引用
收藏
页码:179 / 185
页数:7
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